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Saliva also contains a large number of bacteria, but the existence of a true indigenous salivary microbiota is a matter of debate, as the high flow rate of saliva and low nutrient content would not easily allow bacterial proliferation.
To a large extent, the organisms found in the saliva are those shed by or dislodged from other oral surfaces, in particular the dorsal surface of the tongue .
The microbiota on the dorsal surface of the tongue is more similar among partners than unrelated individuals, but its similarity does not clearly correlate to kissing behavior, suggesting an important role for specific selection mechanisms resulting from a shared lifestyle, environment, or genetic factors from the host.
On average, 96 ± 28 OTUs were observed per oral sample.
After taxonomic classification, we depicted the 15 most abundant genera present in the oral microbiota and the yoghurt drink in Figure Study design and top 15 abundant genera of the oral microbiota and probiotic yoghurt.
A total number of 3,000 OTUs, based on 97% sequence percent similarity, were identified in the tongue and saliva samples (Additional file ).
Only 25 of these OTUs were observed in more than 50% of all 284 oral microbiota samples.